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Wikipedia

GADD45A

Septiembre 10, 2021

La proteína de arresto de crecimiento e inducible por daños en el ADN GADD45 alfa es una proteína que en humanos está codificada por el gen GADD45A.[1]​[2]​[3]​

Función

Este gen es un miembro de un grupo de genes, los genes GADD45, cuyos niveles de transcripción aumentan luego de condiciones estresantes de detención de crecimiento y tratamiento con agentes que dañan el ADN (mutágenos). La transcripción inducida por el daño en el ADN de este gen está mediada por mecanismos dependientes e independientes de p53. La proteína codificada por este gen responde al estrés ambiental al mediar la activación de la vía p38/JNK a través de la quinasa MTK1/MEKK4.[3]​

Aplicaciones

Se ha explotado el hecho de que la expresión de este gen sea un indicador de daño en el ADN para poner a punto una prueba in vitro de mutagenicidad, el ensayo GreenScreen HC de GADD45a-GFP.[4]​ Este ensayo consiste en una línea celular que se ha diseñado para que la expresión de GADD45A esté asociada a la expresión de la proteína verde fluorescente, que puede detectarse fácilmente. Para probar la mutagenicidad de una sustancia, se aplica a estas células y se mide la fluorescencia.

Interacciones

Se ha demostrado que GADD45A interactúa con:

Véase también

  • Gadd45

Referencias

  1. «Induction by ionizing radiation of the gadd45 gene in cultured human cells: lack of mediation by protein kinase C». Mol Cell Biol 11 (2): 1009-16. March 1991. PMC 359769. PMID 1990262. 
  2. «Analysis of the mammalian gadd45 gene and its response to DNA damage». J Biol Chem 268 (32): 24385-93. December 1993. PMID 8226988. 
  3. ↑ «Entrez Gene: GADD45A growth arrest and DNA-damage-inducible, alpha». 
  4. «The GADD45a-GFP GreenScreen HC assay». Methods Mol. Biol. 817: 231-50. 2012. PMID 22147576. doi:10.1007/978-1-61779-421-6_12. 
  5. ↑ «Solution structure of human growth arrest and DNA damage 45alpha (Gadd45alpha) and its interactions with proliferating cell nuclear antigen (PCNA) and Aurora A kinase». J. Biol. Chem. 285 (29): 22196-201. July 2010. PMC 2903397. PMID 20460379. doi:10.1074/jbc.M109.069344. 
  6. ↑ «Association with Cdc2 and inhibition of Cdc2/Cyclin B1 kinase activity by the p53-regulated protein Gadd45». Oncogene 18 (18): 2892-900. May 1999. PMID 10362260. doi:10.1038/sj.onc.1202667. 
  7. «The GADD45 inhibition of Cdc2 kinase correlates with GADD45-mediated growth suppression». J. Biol. Chem. 275 (22): 16602-8. June 2000. PMID 10747892. doi:10.1074/jbc.M000284200. 
  8. ↑ «Identification of a functional domain in a GADD45-mediated G2/M checkpoint». J. Biol. Chem. 275 (47): 36892-8. November 2000. PMID 10973963. doi:10.1074/jbc.M005319200. 
  9. ↑ «GADD45b and GADD45g are cdc2/cyclinB1 kinase inhibitors with a role in S and G2/M cell cycle checkpoints induced by genotoxic stress». J. Cell. Physiol. 192 (3): 327-38. September 2002. PMID 12124778. doi:10.1002/jcp.10140. 
  10. «CR6-interacting factor 1 interacts with Gadd45 family proteins and modulates the cell cycle». J. Biol. Chem. 278 (30): 28079-88. July 2003. PMID 12716909. doi:10.1074/jbc.M212835200. 
  11. «A family of stress-inducible GADD45-like proteins mediate activation of the stress-responsive MTK1/MEKK4 MAPKKK». Cell 95 (4): 521-30. November 1998. PMID 9827804. doi:10.1016/s0092-8674(00)81619-0. 
  12. «The central region of Gadd45 is required for its interaction with p21/WAF1». Exp. Cell Res. 258 (1): 92-100. July 2000. PMID 10912791. doi:10.1006/excr.2000.4906. 
  13. «Interaction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen». Science 266 (5189): 1376-80. November 1994. PMID 7973727. doi:10.1126/science.7973727. 
  14. «Direct interaction of Gadd45 with PCNA and evidence for competitive interaction of Gadd45 and p21Waf1/Cip1 with PCNA». Oncogene 11 (10): 1931-7. November 1995. PMID 7478510. 
  15. «Characterization of MyD118, Gadd45, and proliferating cell nuclear antigen (PCNA) interacting domains. PCNA impedes MyD118 AND Gadd45-mediated negative growth control». J. Biol. Chem. 275 (22): 16810-9. June 2000. PMID 10828065. doi:10.1074/jbc.275.22.16810. 
  16. «Characterisation of the interaction between PCNA and Gadd45». Oncogene 10 (12): 2427-33. June 1995. PMID 7784094. 

Bibliografía

  • Hildesheim J, Fornace AJ (2003). «Gadd45a: an elusive yet attractive candidate gene in pancreatic cancer.». Clin. Cancer Res. 8 (8): 2475-9. PMID 12171872. 
  • Chen IT, Smith ML, O'Connor PM, Fornace AJ (1995). «Direct interaction of Gadd45 with PCNA and evidence for competitive interaction of Gadd45 and p21Waf1/Cip1 with PCNA.». Oncogene 11 (10): 1931-7. PMID 7478510. 
  • Kearsey JM, Coates PJ, Prescott AR (1995). «Gadd45 is a nuclear cell cycle regulated protein which interacts with p21Cip1.». Oncogene 11 (9): 1675-83. PMID 7478594. 
  • Hall PA, Kearsey JM, Coates PJ (1995). «Characterisation of the interaction between PCNA and Gadd45.». Oncogene 10 (12): 2427-33. PMID 7784094. 
  • Carrier F, Smith ML, Bae I (1995). «Characterization of human Gadd45, a p53-regulated protein.». J. Biol. Chem. 269 (51): 32672-7. PMID 7798274. 
  • Smith ML, Chen IT, Zhan Q (1994). «Interaction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen.». Science 266 (5189): 1376-80. PMID 7973727. doi:10.1126/science.7973727. 
  • Warbrick E, Lane DP, Glover DM, Cox LS (1997). «Homologous regions of Fen1 and p21Cip1 compete for binding to the same site on PCNA: a potential mechanism to co-ordinate DNA replication and repair.». Oncogene 14 (19): 2313-21. PMID 9178907. doi:10.1038/sj.onc.1201072. 
  • Warbrick E, Heatherington W, Lane DP, Glover DM (1998). «PCNA binding proteins in Drosophila melanogaster : the analysis of a conserved PCNA binding domain.». Nucleic Acids Res. 26 (17): 3925-32. PMC 147798. PMID 9705499. doi:10.1093/nar/26.17.3925. 
  • Takekawa M, Saito H (1998). «A family of stress-inducible GADD45-like proteins mediate activation of the stress-responsive MTK1/MEKK4 MAPKKK.». Cell 95 (4): 521-30. PMID 9827804. doi:10.1016/S0092-8674(00)81619-0. 
  • Carrier F, Georgel PT, Pourquier P (1999). «Gadd45, a p53-responsive stress protein, modifies DNA accessibility on damaged chromatin.». Mol. Cell. Biol. 19 (3): 1673-85. PMC 83961. PMID 10022855. 
  • Zhan Q, Antinore MJ, Wang XW (1999). «Association with Cdc2 and inhibition of Cdc2/Cyclin B1 kinase activity by the p53-regulated protein Gadd45.». Oncogene 18 (18): 2892-900. PMID 10362260. doi:10.1038/sj.onc.1202667. 
  • Jin S, Antinore MJ, Lung FD (2000). «The GADD45 inhibition of Cdc2 kinase correlates with GADD45-mediated growth suppression.». J. Biol. Chem. 275 (22): 16602-8. PMID 10747892. doi:10.1074/jbc.M000284200. 
  • Vairapandi M, Azam N, Balliet AG (2000). «Characterization of MyD118, Gadd45, and proliferating cell nuclear antigen (PCNA) interacting domains. PCNA impedes MyD118 AND Gadd45-mediated negative growth control.». J. Biol. Chem. 275 (22): 16810-9. PMID 10828065. doi:10.1074/jbc.275.22.16810. 
  • Yi YW, Kim D, Jung N (2000). «Gadd45 family proteins are coactivators of nuclear hormone receptors.». Biochem. Biophys. Res. Commun. 272 (1): 193-8. PMID 10872826. doi:10.1006/bbrc.2000.2760. 
  • Zhao H, Jin S, Antinore MJ (2000). «The central region of Gadd45 is required for its interaction with p21/WAF1.». Exp. Cell Res. 258 (1): 92-100. PMID 10912791. doi:10.1006/excr.2000.4906. 
  • Yang Q, Manicone A, Coursen JD (2001). «Identification of a functional domain in a GADD45-mediated G2/M checkpoint.». J. Biol. Chem. 275 (47): 36892-8. PMID 10973963. doi:10.1074/jbc.M005319200. 
  • Hartley JL, Temple GF, Brasch MA (2001). «DNA cloning using in vitro site-specific recombination.». Genome Res. 10 (11): 1788-95. PMC 310948. PMID 11076863. doi:10.1101/gr.143000. 
  • Kovalsky O, Lung FD, Roller PP, Fornace AJ (2001). «Oligomerization of human Gadd45a protein.». J. Biol. Chem. 276 (42): 39330-9. PMID 11498536. doi:10.1074/jbc.M105115200. 
  •   Datos: Q17913576
  •   Multimedia: GADD45A

Septiembre 10, 2021
gadd45a, proteína, arresto, crecimiento, inducible, daños, gadd45, alfa, proteína, humanos, está, codificada, Índice, función, aplicaciones, interacciones, véase, también, referencias, bibliografíafunción, editareste, miembro, grupo, genes, genes, gadd45, cuyo. La proteina de arresto de crecimiento e inducible por danos en el ADN GADD45 alfa es una proteina que en humanos esta codificada por el gen GADD45A 1 2 3 Indice 1 Funcion 2 Aplicaciones 3 Interacciones 4 Vease tambien 5 Referencias 6 BibliografiaFuncion EditarEste gen es un miembro de un grupo de genes los genes GADD45 cuyos niveles de transcripcion aumentan luego de condiciones estresantes de detencion de crecimiento y tratamiento con agentes que danan el ADN mutagenos La transcripcion inducida por el dano en el ADN de este gen esta mediada por mecanismos dependientes e independientes de p53 La proteina codificada por este gen responde al estres ambiental al mediar la activacion de la via p38 JNK a traves de la quinasa MTK1 MEKK4 3 Aplicaciones EditarSe ha explotado el hecho de que la expresion de este gen sea un indicador de dano en el ADN para poner a punto una prueba in vitro de mutagenicidad el ensayo GreenScreen HC de GADD45a GFP 4 Este ensayo consiste en una linea celular que se ha disenado para que la expresion de GADD45A este asociada a la expresion de la proteina verde fluorescente que puede detectarse facilmente Para probar la mutagenicidad de una sustancia se aplica a estas celulas y se mide la fluorescencia Interacciones EditarSe ha demostrado que GADD45A interactua con AURKA 5 5 Cdk1 6 7 8 9 CCNB1 6 9 GADD45GIP1 10 MAP3K4 11 P21 8 12 y PCNA 8 13 14 15 16 Vease tambien EditarGadd45Referencias Editar Induction by ionizing radiation of the gadd45 gene in cultured human cells lack of mediation by protein kinase C Mol Cell Biol 11 2 1009 16 March 1991 PMC 359769 PMID 1990262 Analysis of the mammalian gadd45 gene and its response to DNA damage J Biol Chem 268 32 24385 93 December 1993 PMID 8226988 a b Entrez Gene GADD45A growth arrest and DNA damage inducible alpha The GADD45a GFP GreenScreen HC assay Methods Mol Biol 817 231 50 2012 PMID 22147576 doi 10 1007 978 1 61779 421 6 12 a b Solution structure of human growth arrest and DNA damage 45alpha Gadd45alpha and its interactions with proliferating cell nuclear antigen PCNA and Aurora A kinase J Biol Chem 285 29 22196 201 July 2010 PMC 2903397 PMID 20460379 doi 10 1074 jbc M109 069344 a b Association with Cdc2 and inhibition of Cdc2 Cyclin B1 kinase activity by the p53 regulated protein Gadd45 Oncogene 18 18 2892 900 May 1999 PMID 10362260 doi 10 1038 sj onc 1202667 The GADD45 inhibition of Cdc2 kinase correlates with GADD45 mediated growth suppression J Biol Chem 275 22 16602 8 June 2000 PMID 10747892 doi 10 1074 jbc M000284200 a b c Identification of a functional domain in a GADD45 mediated G2 M checkpoint J Biol Chem 275 47 36892 8 November 2000 PMID 10973963 doi 10 1074 jbc M005319200 a b GADD45b and GADD45g are cdc2 cyclinB1 kinase inhibitors with a role in S and G2 M cell cycle checkpoints induced by genotoxic stress J Cell Physiol 192 3 327 38 September 2002 PMID 12124778 doi 10 1002 jcp 10140 CR6 interacting factor 1 interacts with Gadd45 family proteins and modulates the cell cycle J Biol Chem 278 30 28079 88 July 2003 PMID 12716909 doi 10 1074 jbc M212835200 A family of stress inducible GADD45 like proteins mediate activation of the stress responsive MTK1 MEKK4 MAPKKK Cell 95 4 521 30 November 1998 PMID 9827804 doi 10 1016 s0092 8674 00 81619 0 The central region of Gadd45 is required for its interaction with p21 WAF1 Exp Cell Res 258 1 92 100 July 2000 PMID 10912791 doi 10 1006 excr 2000 4906 Interaction of the p53 regulated protein Gadd45 with proliferating cell nuclear antigen Science 266 5189 1376 80 November 1994 PMID 7973727 doi 10 1126 science 7973727 Direct interaction of Gadd45 with PCNA and evidence for competitive interaction of Gadd45 and p21Waf1 Cip1 with PCNA Oncogene 11 10 1931 7 November 1995 PMID 7478510 Characterization of MyD118 Gadd45 and proliferating cell nuclear antigen PCNA interacting domains PCNA impedes MyD118 AND Gadd45 mediated negative growth control J Biol Chem 275 22 16810 9 June 2000 PMID 10828065 doi 10 1074 jbc 275 22 16810 Characterisation of the interaction between PCNA and Gadd45 Oncogene 10 12 2427 33 June 1995 PMID 7784094 Bibliografia EditarHildesheim J Fornace AJ 2003 Gadd45a an elusive yet attractive candidate gene in pancreatic cancer Clin Cancer Res 8 8 2475 9 PMID 12171872 Chen IT Smith ML O Connor PM Fornace AJ 1995 Direct interaction of Gadd45 with PCNA and evidence for competitive interaction of Gadd45 and p21Waf1 Cip1 with PCNA Oncogene 11 10 1931 7 PMID 7478510 Kearsey JM Coates PJ Prescott AR 1995 Gadd45 is a nuclear cell cycle regulated protein which interacts with p21Cip1 Oncogene 11 9 1675 83 PMID 7478594 Hall PA Kearsey JM Coates PJ 1995 Characterisation of the interaction between PCNA and Gadd45 Oncogene 10 12 2427 33 PMID 7784094 Carrier F Smith ML Bae I 1995 Characterization of human Gadd45 a p53 regulated protein J Biol Chem 269 51 32672 7 PMID 7798274 Smith ML Chen IT Zhan Q 1994 Interaction of the p53 regulated protein Gadd45 with proliferating cell nuclear antigen Science 266 5189 1376 80 PMID 7973727 doi 10 1126 science 7973727 Warbrick E Lane DP Glover DM Cox LS 1997 Homologous regions of Fen1 and p21Cip1 compete for binding to the same site on PCNA a potential mechanism to co ordinate DNA replication and repair Oncogene 14 19 2313 21 PMID 9178907 doi 10 1038 sj onc 1201072 Warbrick E Heatherington W Lane DP Glover DM 1998 PCNA binding proteins in Drosophila melanogaster the analysis of a conserved PCNA binding domain Nucleic Acids Res 26 17 3925 32 PMC 147798 PMID 9705499 doi 10 1093 nar 26 17 3925 Takekawa M Saito H 1998 A family of stress inducible GADD45 like proteins mediate activation of the stress responsive MTK1 MEKK4 MAPKKK Cell 95 4 521 30 PMID 9827804 doi 10 1016 S0092 8674 00 81619 0 Carrier F Georgel PT Pourquier P 1999 Gadd45 a p53 responsive stress protein modifies DNA accessibility on damaged chromatin Mol Cell Biol 19 3 1673 85 PMC 83961 PMID 10022855 Zhan Q Antinore MJ Wang XW 1999 Association with Cdc2 and inhibition of Cdc2 Cyclin B1 kinase activity by the p53 regulated protein Gadd45 Oncogene 18 18 2892 900 PMID 10362260 doi 10 1038 sj onc 1202667 Jin S Antinore MJ Lung FD 2000 The GADD45 inhibition of Cdc2 kinase correlates with GADD45 mediated growth suppression J Biol Chem 275 22 16602 8 PMID 10747892 doi 10 1074 jbc M000284200 Vairapandi M Azam N Balliet AG 2000 Characterization of MyD118 Gadd45 and proliferating cell nuclear antigen PCNA interacting domains PCNA impedes MyD118 AND Gadd45 mediated negative growth control J Biol Chem 275 22 16810 9 PMID 10828065 doi 10 1074 jbc 275 22 16810 Yi YW Kim D Jung N 2000 Gadd45 family proteins are coactivators of nuclear hormone receptors Biochem Biophys Res Commun 272 1 193 8 PMID 10872826 doi 10 1006 bbrc 2000 2760 Zhao H Jin S Antinore MJ 2000 The central region of Gadd45 is required for its interaction with p21 WAF1 Exp Cell Res 258 1 92 100 PMID 10912791 doi 10 1006 excr 2000 4906 Yang Q Manicone A Coursen JD 2001 Identification of a functional domain in a GADD45 mediated G2 M checkpoint J Biol Chem 275 47 36892 8 PMID 10973963 doi 10 1074 jbc M005319200 Hartley JL Temple GF Brasch MA 2001 DNA cloning using in vitro site specific recombination Genome Res 10 11 1788 95 PMC 310948 PMID 11076863 doi 10 1101 gr 143000 Kovalsky O Lung FD Roller PP Fornace AJ 2001 Oligomerization of human Gadd45a protein J Biol Chem 276 42 39330 9 PMID 11498536 doi 10 1074 jbc M105115200 Datos Q17913576 Multimedia GADD45AObtenido de https es wikipedia org w index php title GADD45A amp oldid 120772499, wikipedia, wiki, leyendo, leer, libro, biblioteca,

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